2024 Fth1 abcam

Fth1 abcam

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August undefined, 2024

WebFTH1 antibody; FTH1 protein antibody; FTHL 6 antibody; FTHL6 antibody; Iron overload autosomal dominant antibody; MGC104426 antibody; N … WebDec 1, 2024 · Subsequently, we showed that ferritin heavy chain 1 (FTH1) was a key determinant for baicalin-induced ferroptosis. Overexpression of FTH1 abrogated the anticancer effects of baicalin in bladder cancer cells. Taken together, our data for the first time suggest that baicalin exerts its anticancer function by triggering FTH1-dependent …

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WebSep 22, 2024 · Overexpression of ferritin heavy chain (FTH1) often associates with good prognosis in breast cancer (BCa), particularly in the triple-negative subtype (triple-negative breast cancer).However, the mechanism by which FTH1 exerts its possible tumor suppressor effects in BCa is not known. Here, we examined the bearing of FTH1 … WebFeb 20, 2024 · H-ferritin is not necessary for in vitro differentiation of bone marrow-derived macrophages. (a) Quantification of FTH1 and FTL by Western blot in protein extracts from Fth1 +/+ (black) and Fth1 ... magnolia nursing facility

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WebApr 4, 2024 · In addition, ANXA1sp considerably eliminated LPS-caused H9C2 cell ferroptosis, as revealed by the suppression of iron accumulation and the increase in GPX4 and FTH1 expression. WebOct 25, 2024 · According to the instructions, the expression levels of ferritin FTL (ab155517, Abcam) and FTH1 (ab78877, Abcam) in the retina were detected using the corresponding kits. Immunohistochemistry (IHC) WebMay 29, 2024 · The expression of FTH1 decreased significantly in the Pacing group, which is consistent with the results of our vivo experiments, and Fer-1 alleviated the degradation of FTH1, GPX4, and SLC7A11 (Figures 4(g) and 4(h)). Hence, cardiomyocytes may initially resist to rapid pacing-induced ferroptosis. magnolia nursing home clinton nc

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WebFeb 2, 2024 · Ferritin is a ubiquitously expressed cytosolic heteropolymer composed of H-chains (FTH1) and L-chains (FTL) ... (Abcam, ab7475) to detect LC3B-FTH1 co-localization and with 10% normal donkey serum and 10% normal goat serum (Abcam, ab7481) to detect LAMP2a-FTH1 co-localization. The primary antibodies were rabbit anti-FTH1, … WebMar 27, 2024 · The membranes were incubated overnight at 4 °C with the following primary antibodies: ACSL4 (Abcam), GPx4 (Abcam), FTH1 (Abcam), COX2 (Abcam), and β-actin (Sigma). Finally, the membranes were incubated with corresponding secondary antibodies for 90 min at room temperature, and densitometry measurements of the western blot … magnolia nursing home greenville scWebDec 21, 2024 · PAS staining was also employed for morphologic evaluation using Periodic Acid Schiff Staining Kit (Abcam, catalog # ab150680). The sections were incubated with periodic acid solution for 10 min after de-paraffinization and rehydration. ... It is noteworthy that FTH1 was up-regulated in cisplatin-treated kidneys, unlike the changes in BUMPT ... magnolia nursing home gardendale al

"WebMar 21, 2024 · FTH1 (Ferritin Heavy Chain 1) is a Protein Coding gene. Diseases associated with FTH1 include Hemochromatosis, Type 5 and Iron Overload . Among its … " - Fth1 abcam

Fth1 abcam

H-Ferritin is essential for macrophages’ capacity to store …

WebSep 21, 2024 · FTH1 plays an important role in the maintenance of the cellular iron balance in ferroptosis. Interestingly, FTH1 was also found to be involved in ferritinophagy, a … WebFeb 10, 2024 · Cell Signaling Technology's FTH1 (D1D4) Rabbit mAb is a Rabbit monoclonal antibody. The FTH1 (D1D4) Rabbit mAb Antibody was generated using FTH1 as the antigen. It reacts with Human, Mouse, Rat, and Non-Human Primate. This antibody has been shown to work in applications such as: Precipitation, Immunohistochemistry, …

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WebAbcam catalog: ab183781 total number of products: 13. domestic rabbit monoclonal (EPR18878) reactivity: human, mouse, rat application: western blot, immunohistochemistry - paraffin section ... The membrane was incubated with rabbit anti-FTH1 monoclonal antibody (Catalog # M02401) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 ... WebFeb 20, 2024 · Bone marrow-derived macrophages (BMDM) were obtained from Fth1 Fl/Fl; Lyz2 +/+ (Fth1 +/+, H-ferritin-sufficient) and Fth1 Fl/Fl; Lyz2 cre/+ (Fth1 −/−, H-ferritin …

Web1.1 主要试剂及仪器(1)主要试剂:Delphinidin、DFP(美国Sigma公司)，胎牛血清(美国Gibco公司)，DMEM高糖培养基(美国Hyclone公司)，CCK-8试剂盒(日本同仁公司)，细胞凋亡与坏死检测试剂盒(中国碧云天公司)，钙黄绿素-乙酰羟甲基酯荧光探针(Calcein-AM，英国Abcam公司)，BODIPYTM 581 ... WebJul 1, 2024 · The antibodies used were anti-GAPDH (Cell Signaling, 5174), anti-GPX4 (abcam, ab125066), anti-FTH1 (abcam, ab65080), anti-Transferrin (abcam, ab82411), ... FTH1 is the heavy subunit of ferritin, which plays an essential role in maintaining the balance of iron metabolism in cells.

WebNov 5, 2024 · For detection of FTH1, the membrane was blocked with 5% bovine serum albumin (BSA; Beyotime) for 1 h and then incubated with a primary antibody that specifically recognized FTH1 (rabbit anti-FTH1, 1:1000; Abcam, Cambridge, England) or GAPDH (rabbit anti-FTH1, Abcam) overnight at 4°C. WebSep 3, 2024 · Abcam FTH1 antibody (Abcam, ab75973) was used in immunohistochemistry - paraffin section on mouse samples at 1:200 (fig 4f) and in western blot on mouse samples at 1:3000 (fig 4e). Antioxidants (Basel) (2024) ncbi: domestic rabbit polyclonal. ab65080;

WebSupplementary Figure 3: Quality control of single-cell RNA-seq data and clustering of human chondrocytes.(A) Number of UMI, number of features, percent of mitochondrial gene and log10GenesPerUMI distribution of human chondrocytes in each of cartilage sample.(B) Visualize the correlation between the number of detected genes and the number of …

WebHHMI’s Janelia Research Campus in Ashburn, Virginia, cracks open scientific fields by breaking through technical and intellectual barriers. Our integrated teams of lab scientists … magnolia nursing home ncWebFeb 11, 2024 · Seventy percent of the world’s internet traffic passes through all of that fiber. That’s why Ashburn is known as Data Center Alley. The Silicon Valley of the east. The … magnolia nutrition gonzales laWeb丁香通为您提供pComb3XLambd商品详情介绍：价格：￥14，货号：P49004，CAS号：无，品牌：禾午生物，产地：国产，供应商：禾午生物，详见丁香通pComb3XLambd商品详情页； magnolia nutrition columbus msWebThe cells were then blocked with 5% BSA for 1 h at 37 °C and treated overnight at 4 °C with Parkin (1:200) and LC3B (#ab192890 Abcam, Cambridge, United Kingdom 1:500) primary antibodies. After three washes with PBS, cells were incubated for 1 h at 37 °C in the dark with FITC-conjugated goat anti-rabbit secondary antibody (A0562, Beyotime ... cra2-150nWebThe results of qRT-PCR and Western blot experimental verification indicated that the expression patterns of FTH1, except for ATP6V1A, were similar with those predicted by bioinformatics analysis. ... ab180649), GPX4 (Abcam, ab125066), NOX1 (Abcam, ab131088), ACSL4 (Abcam, ab155282), FTH1 (Abcam, ab183781), ATP6V1A (Abcam, … cra22 cra 2022 disability tax creditWebThe FTH1 Antibody from Cell Signaling Technology is a Rabbit Polyclonal antibody to FTH1. This antibody recognizes Human, Mouse, Rat, and Non-Human Primate antigen. The FTH1 Antibody has been validated for the following applications: Western Blot. FTH1 Antibody detects endogenous levels of total FTH1 protein. Nonspecific bands are seen above 80 ... cra 228.43