Gold antifade reagent
WebJul 25, 2006 · Their product, ProLong® Gold, is a ready-to-use antifade reagent that significantly reduces bleaching and allows experiments to be saved for months after mounting. This antifade reagent cures to … WebSamples were mounted in ProLong ® Gold Antifade Reagent with DAPI #8961 (blue). Data were generated using the standard formulation of this product. Show Less Show More. Confocal immunofluorescent analysis of mouse retina using Neurogranin (E9L8D) Rabbit mAb (green), GFAP (GA5) Mouse mAb (Alexa Fluor ...
Gold antifade reagent
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WebJan 3, 2013 · antifade reagents. ProLong ® Gold antifade reagent (P36930, P36934) offers enhanced resistance to photobleaching and is premixed and ready to use—just add a drop of reagent and mount. ProLong ® Gold reagent causes little or no quenching of the fluorescent signal (Figure 1). Like WebProLong Gold Antifade is specifically designed to help protect fluorescent dyes (i.e. Alexa Fluor) from fading but is not recommended with fluorescent proteins (GFP, RFP, mCherry). It comes ready-to-use and stored at room temperature—just apply a drop on the sample, add a coverslip, cure, and image. Supplier: Invitrogen™ P36930
WebThe only advantage of using the Prolong gold antifade mounting reagent with DAPI is to save you the pain of adding DAPI with your secondary antibodies... So the advantage is quite limited. If... WebProLong™ Live Antifade Reagent 4 Before you begin Storage and handling ProLong™ Live reagent can be stored at 2–8°C for up to 30 days. For longer storage, store the …
WebI have used Prolong Gold Antifade with DAPI on cytospun cells or frozen human skeletal muscle tissue sections, 5 µm thick, fixed with either acetone or formaldehyde w/ or w/o triton-x as...
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WebAntifade Reagents provide protection against fading or photobleaching for most common fluorophores used in live and fixed cell fluorescent imaging. Products. Background. Literature (1) Cat. No. Product Name / Activity. 4055. L -Ascorbic acid. Commonly used antifade reagent in live cell microscopy; naturally occurring antioxidant. how to look more tannedWebMounting media comprise two or three main ingredients: a base, an antifade reagent, and sometimes a plasticizer to set. * This information is a collation of useful comments from the Confocal listserver archives (CLA) ... joined by Prolong Gold (which comes premixed but is more expensive per ml.). Prolong is joules white giletWebJul 25, 2006 · Their product, ProLong® Gold, is a ready-to-use antifade reagent that significantly reduces bleaching and allows experiments to be saved for months after mounting. This antifade reagent cures to permanently fix coverslips in place, which prevents coverslips from moving around and destroying objectives with media. how to look more sophisticatedWebJan 17, 2024 · For confocal microscopic analysis, antibodies stained samples were mounted with fluorescence mounting medium (ProLong Gold Antifade Reagent, ThermoFisher Scientific, OR, USA) and images were obtained with a FV1000 or FV3000 laser-scanning confocal microscope (Olympus, Tokyo, Japan). ASW2.1 and FV31S software (Olympus) … joules women\u0027s clothing saleWebSamples were mounted in ProLong ® Gold Antifade Reagent with DAPI #8961 (blue). Show Less Show More Confocal immunofluorescent analysis of A549 cells transiently transfected with an mCherry construct, using … joules women\u0027s sweatshirtsWebProLong ® Gold Antifade Reagent is stable for 12 months when stored, protected from light, at or below -20°C. Background ProLong ® Gold … how to look my ageWebNov 25, 2024 · ProLong® Gold reagent is an antifade liquid mountant used for coverslipping fluorescently labeled cell or tissue samples on microscope slides. This mounting media can be purchased with or without DAPI. DAPI (4',6-diamidino-2-phenylindole) can ne thought of as a counterstain that labels the nuclei in a sample. … joules women\\u0027s wear